1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit, 96 well plate

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About product

Catalog number:EKU08306
Name:1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit, 96 well plate
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Extra details

Verified reactivity:Multispecies (General)
Detection limits:123.5-10000 pg/mL
Sensitivity limit:49,5 pg/mL
Samples to be analyzed:Serum, plasma and other biological fluids
Assay duration:2 hours
Verified applications:ELISA
Assay class:Competitive
ELISA detection:Colorimetric
Protein number:Please refer to Uniprot
Alternate protein number:Please refer to SwissProt
Gene number:Please refer to GenBank
Gene name:1,25-Dihydroxyvitamin D3
Alternate gene name:DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
Estimated production time:7-11 business days
Shipping requirements:Blue ice
Storage recommendation:-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for:8 months
ELISA's specificity:This assay has high sensitivity and excellent specificity for detection of 1,25-Dihydroxyvitamin D3 (DHVD3). No significant cross-reactivity or interference between 1,25-Dihydroxyvitamin D3 (DHVD3) and analogues was observed.
ELISA's cross-reactivity:This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,25-Dihydroxyvitamin D3 (DHVD3) and unlabeled 1,25-Dihydroxyvitamin D3 (DHVD3) (Standards or samples) with the pre-coated antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,25-Dihydroxyvitamin D3 (DHVD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,25-Dihydroxyvitamin D3 (DHVD3) in the sample.
Protocol:Please see ELISA's datasheet, otherwise contact us
Precision of the test:Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability:The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC:The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area:Metabolic pathway;Bone metabolism;
Warnings:The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes:For research use only. Not for diagnostic procedures.
Properties:E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test:ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description:A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

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