Anti-Bromodeoxyuridine (Brdu), Biotin, (Clone: BU1/75 (ICR1)), (Rat IgG2a)

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About product

Catalog number:GEN520517
Name:Anti-Bromodeoxyuridine (Brdu), Biotin, (Clone: BU1/75 (ICR1)), (Rat IgG2a)
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Extra details

Also known as:Bromodeoxyuridine (Brdu)
Other names:N/A
Subcategory:Mnoclonal antibodies
Gene name:N/A
Gene name synonims:N/A
Other gene names:N/A
Immunoglobulin isotype:Rat IgG2a
Clone:BU1/75 (ICR1)
Host organism:Rat (Rattus norvegicus)
Species reactivity:N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity:Bromodeoxyuridine (Brdu) (Reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. It also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not cross react with thymidine); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Purification method:Protein G
Form/Appearance:Biotin (Purified Ig in PBS/Glycerol (50%) + BSA (10 mg/ml))
Storage and shipping:Store the antibody at +4 degrees Celsius. for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius. for longer periods. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided.
Tested applications::Immunocytochemistry (ICC)
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
About:Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name:Rattus norvegicus

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