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1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based)

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Catalog number:K684-50
Name:1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based)
Size:50 assays
Price:885.00
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Kit's description:1,5-Anhydroglucitol (1,5-AG) is the 1-deoxy form of D-glucose. Its level in blood plasma has been used in clinical research to investigate short-term glycemic control levels in diabetic patients. Cereals and animal source proteins constitute the main 1,5-AG source for humans. Most of 1,5-AG is absorbed by renal tubules, and its levels in human body are highly regulated and relatively consistent. Glucose can act as a competitive inhibitor of 1,5-AG in blood. Therefore, glucose levels are inversely proportional to 1,5-AG concentrations. The physiological function of 1,5-AG is not well understood. Recent studies showed 1,5-AG uptake could be associated with sodium-dependent glucose transporter. BioVision’s 1,5-Anhydroglucitol Uptake Assay Kit utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells
Highlights:• Detection method: FACS (488 nm excitation laser) and fluorescent microscope (excitation range 420 nm:495 nm) • Application: Measurement of 1,5:Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. : Dual:staining of 1,5:AG transporters and 1,5:AG uptake : Analysis of 1,5:AG metabolism and cell signaling in various cell types : Screening of anti:diabetic compounds
Kit's benefits:• Easy-to-use • Non-radioactive • Image and accurately measure 1,5-Anhydroglucitol uptake in cultured cells in response to insulin, growth factors etc.
Category:Metabolism, 1,5-AG uptake assay
Kit's other name:none
Contents:• Analysis Buffer • AGTrackerTM Reagent (100X) • Phloretin (100X) (in DMSO) • Phloretin (100X)
Storage condition:-20°C
Shipping condition:gel pack
Maximum time can be stored:12 months
Species reactivity:The most sensitive FACS kit
Applications:• Measurement of 1,5-Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. • Dual-staining of 1,5-AG transporters and 1,5-AG uptake • Analysis of 1,5-AG metabolism and cell signaling in various cell types • Screening of anti-diabetic compounds
Samples tested:Adherent or suspension cells
Test:Biovision supplies other types of Assays as 1.
Description:For cells, cell lines and tissues in culture till half confluency.

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