AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)

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About product

Catalog number:VPK-417-DJ-8
Name:AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)
Size:1 test kit
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Extra details

We send this reagant on:Blue Ice
The temperatureerature for storage is:-20ºC
SDS or safty data sheet: (VPK-410 through VPK-430-SER6) GHS.pdf
Type of reagent:Viral Expression
Related techniques:Viral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-DJ/8 Helper Free Complete Expression Systems
Use of the reagent:The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
Cellular use:Complete Expression System contains packaging plasmids, expression vector and GFP control vector
Protocol information:pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
Reagent properties:AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
Additional lab advice:Cloning capacity of expression vector = 1.4 kb
Living modified organisme in this reagent or LMO:Not applicable
Species compatible to:Humans
Use of this test kit or product:Genetics lab
Antigen:a specific protein sequence
Buffer:The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,33
Molecular weight:in kilo Danlto or kDa
This reagent is purified:0.97

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