96-Well ROCK Activity Assay test kit

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About product

Catalog number:STA-416-5
Name:96-Well ROCK Activity Assay test kit
Size:5 x 96 assays
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Extra details

We send this reagant on:Blue Ice
The temperatureerature for storage is:Store active ROCK-II at -80ºC, ATP Solution at -20ºC and all other test kit components at 4ºC. Avoid multiple freeze/thaw cycles.
SDS or safty data sheet: (STA-416) GHS.pdf
Type of reagent:Cell Signaling and Protein Biology
Related techniques:Cell Signaling and Protein Biology, Kinase Assays, Rho Kinase (ROCK) Assays
Use of the reagent:Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay test kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The test kit contains a strip-well plate pre-coated with recombinant MYPT1.
Cellular use:Safe non-radioactive ELISA format
Protocol information:Active ROCK-II included as positive control
Living modified organisme in this reagent or LMO:Recombinant Protein from Baculovirus
Species compatible to:Humans
Use of this test kit or product:Genetics lab
Antigen:a specific protein sequence
Buffer:The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,2
Molecular weight:in kilo Danlto or kDa
This reagent is purified:0.97
Test:Cellular biology labs supplies other types of Assays as 1.

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