View details of this awesome product

About product

Catalog number:30 516 401
Name:HtrA4 ELISA Kit
Size:1 Microplate/96 determinations
Go to shop   

Extra details

ELISA category:ELISA Kit
ELISA detection limits:1.25 - 80 ng/ml HtrA4
ELISA sensitivity:1 ng/ml
ELISA's description:The quantitative assay is based on a sandwich format. A highly specific monoclonal antibody for the HtrA4 is immobilized on the plate. HtrA4 is bound to the immobilized antibody, other components of the sample are removed by aspiration and washing. The analyte is detected in two steps using a secondary biotin-labeled antibody and a highly polymerized streptavidin-peroxidase conjugate. Any reagent excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition od TMB Substrate. The reaction is stopped by adding the Stop Solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concentration of HtrA4 in a sample is determined by interpolation from the standard curve.
Research area interests:Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
Package form:96 well microplate
Reacts with:HtrA4
Tested applications:The system provides a highly sensitive and specific quantitative determination of HtrA4 (80 ng/ml - 1.25 ng/ml) in serum, tissue e.g. placenta and cell culture supernatants.
Preservative materials:If applicable mentioned in the product data sheet
Storage recommendations:6 month
Shipping and storage conditions:ice pack
Long term storage:4 °C
Assay principle:Sandwich-ELISA
Included in the standard package:microplate, buffers, antibody conjugate,standards
Not included in the standard package:Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
Samples to be analyzed:biological fluids, cell culture
PubMed citations:See the datasheet
Technical datasheet:Contact us to receive the datasheet
Representative figures:
Warnings:For Research Use only.
Properties:E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test:ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Other suggested products