BioTeZ Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein Biotin Labelling Kit Kit

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Catalog number:BTBIOK-05
Name:BioTeZ Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein Biotin Labelling Kit Kit
Size:For 5 labels
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Extra details

ELISA category:Biotin Labelling Kit Kit
ELISA detection limits:N/A
ELISA sensitivity:N/A
ELISA's description:The BioTeZ Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product. The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22,4 Å to increase the distance between the target molecule and the biotin groups. In the kit are 5 portions of Sulfo-NHS-LC Biotin reagent. One portion is used for a antibody amount of 1-10 mg or a protein amount of 1-5 mg.
Research area interests:all immuno assays
Package form:Kit
Reacts with:Streptavidin
Tested applications:Biotin Labelling , e.g. for antibodies, proteins etc.; Biotinylated molecules bind very well to Streptavidin or Streptavidin surfaces
Preservative materials:If applicable mentioned in the product data sheet
Storage recommendations:12 month
Shipping and storage conditions:> 0 to +37°C
Long term storage:2-8°C/ -20°C
Assay principle:Biotinylation with Sulfo-NHS-LC of antibody / protein, coupling reaction with target molecules through the reaction with primary amino groups
Included in the standard package:Sulfo-NHS-LC Biotin, biotinylation buffer for the coupling reaction, clean-up column and purification buffer concentrate (10x) for size-exclusion chromatography. All components are included for 5 separate coupling reactions.
Not included in the standard package:Pipettes or pipetting equipment, vials for dilution, distilled or deionised water
Samples to be analyzed:Biotinylation of antibody orprotein
PubMed citations:See the datasheet
Technical datasheet:Contact us to receive the datasheet
Representative figures:No Available
Warnings:For Research Use only.
Properties:If you buy Antibodies supplied by biotez they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description:Antibody labelling kits.
French translation:anticorps

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