Bioxys

Acetylated Lysine Antibody: Biotin

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Catalog number:SMC-153D-BI
Name:Acetylated Lysine Antibody: Biotin
Size:100 µg
Price:380.00
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Extra details

Type:Antibodies, Monoclonal
Detection and sensitivity:Raised in: MouseIsotypeIgG1Reactivity: Species Independent. Bovine albumin and avian histones were tested.SpecificityDetects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
Performance and applications:Tested for: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.PurificationProtein G PurifiedIt should be stoored in: PBS pH7.4, 50% glycerol, 0.09% sodium azideConcentration1 mg/ml
Additional information:Also known as: Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine AntibodyClone Number: 7F8Accession Number: Gene ID: Swiss-Prot: Cellular Localization: NucleusCertificate of Analysis: 1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.References: 1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.Tarriff Code: 12352203UNSPSC Code: Non-hazardousADR Code: Non-hazardousUN Code for transport:
Shipping handling and storage:Tested for: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.PurificationProtein G PurifiedIt should be stoored in: PBS pH7.4, 50% glycerol, 0.09% sodium azideConcentration1 mg/ml Also known as: Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine AntibodyClone Number: 7F8Accession Number: Gene ID: Swiss-Prot: Cellular Localization: NucleusCertificate of Analysis: 1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.References: 1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.Tarriff Code: 12352203UNSPSC Code: Non-hazardousADR Code: Non-hazardousUN Code for transport: is shipped with 0
Properties:If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
French translation:anticorps

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