Bioxys

AHA1 Antibody: Biotin

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Catalog number:SMC-198D-BI
Name:AHA1 Antibody: Biotin
Size:100 µg
Price:360.00
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Type:Antibodies, Monoclonal
Detection and sensitivity:Raised in: MouseIsotypeIgG1 KappaReactivity: Human, MouseSpecificityDetects ~38kDa, can run up to 45kDa on SDS Page. It sees human Aha1, Mouse Aha1 and Mouse Aha2.
Performance and applications:Tested for: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.PurificationProtein G PurifiedIt should be stoored in: PBS pH7.2, 50% glycerol, 0.09% sodium azideConcentration1 mg/ml
Additional information:Also known as: Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 AntibodyClone Number: 4H9-D7Accession Number: NP_036243.1Gene ID: 10598Swiss-Prot: O95433Cellular Localization: Cytoplasm, Endoplasmic ReticulumCertificate of Analysis: 1ug/ml of SMC-198 was sufficient for detection of Aha1 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.References: 1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197. Tarriff Code: 12352203UNSPSC Code: Non-hazardousADR Code: Non-hazardousUN Code for transport:
Shipping handling and storage:Tested for: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.PurificationProtein G PurifiedIt should be stoored in: PBS pH7.2, 50% glycerol, 0.09% sodium azideConcentration1 mg/ml Also known as: Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 AntibodyClone Number: 4H9-D7Accession Number: NP_036243.1Gene ID: 10598Swiss-Prot: O95433Cellular Localization: Cytoplasm, Endoplasmic ReticulumCertificate of Analysis: 1ug/ml of SMC-198 was sufficient for detection of Aha1 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.References: 1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197. Tarriff Code: 12352203UNSPSC Code: Non-hazardousADR Code: Non-hazardousUN Code for transport: is shipped with 0
Properties:If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
French translation:anticorps

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