Bioxys

Mouse Monoclonal IgG Anti-AGE-4 Methylglyoxal modifiied [14B5]

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Catalog number:AGE4-010
Name:Mouse Monoclonal IgG Anti-AGE-4 Methylglyoxal modifiied [14B5]
Size:10 µg
Price:904.00
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Availability:Please contact us to check the availability
Immunogen:Methylglyoxal-modified BSA
Specificity:AGE-4 (Methylglyoxal-modified proteins); in Western Blot no cross-reaction with glucose-modified AGEs (AGE-1), AGE-2 (Glycerolaldehyde-modified), AGE-3 (Glycolaldehyde-modified), AGE-5 (Glyoxal-modified), AGE-6 (3-DG-modified), CEL-BSA, CML-BSA and BSA
Species reactivity:not relevant
Product presentation:Protein G affinity purified monoclonal antibody in PBS with 2% Block Ace as stabilizer and 0.1% Proclin as preservative
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG
Antibody's clone:14B5
Product is supplied as:liquid concentrate
Recommended concentration for use:0,1 µg/ml (WB) and 0,05 µg/ml (ELISA)
Protein concentration:0.25 mg/ml
Positive control:human lens, arteriosclerotic plaques
Tested applications:ELISA, WB
Recommended secondary reagent:As secondary system in ELISA and Western Blot we recommend biotinylated anti-mouse IgG antibody (Art. No. ZU102) in combination with streptavidin-HRPO conjugate (Art. No. ZU054) or streptavidin-alkaline phosphatase (Art. No. ZU051).
Storage method:Store at -20°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Takeuchi M. , Makita Z., Bucala R., Suzuki T., Koike T., and Kameda Y. (2000) Immunological evidence that non-carboxymethyllysine Advanced Glycation End-products are produced from short chain sugars and dicarbonyl compounds in vivo. Mol. Med. 6; 114-125. 2. Takeuchi M., Yanase Y., Matsuura N., Yamagishi S., Kameda Y., Bucala R., and Z. Matika (2001) Immunological detection of a novel Advanced Glycation End-Product. Mol. Med. 7; 783-791. 3. Van Herreweghe F. et al. (2002) Tumor necrosis factor-induced modulation of glyoxalase I activities through phosphorylation by PKA results in cell death and is accompanied by the formation of a specific methylglyoxal-derived AGE.: Proc .Natl. Acad. Sci. U S A.;99(2):949-954. 4. Speer O. et al.(2003) Rapid suppression of mitochondrial permeability transition by methylglyoxal. Role of reversible arginine modification. J. Biol .Chem. 278(37):34757-63.
Tips:*Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Additional isotype:IgG
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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