Mouse Monoclonal Ig Anti-Calretinin [6B3]

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Catalog number:CA130
Name:Mouse Monoclonal Ig Anti-Calretinin [6B3]
Size:1 ml
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Availability:Please contact us to check the availability
Immunogen:Recombinant human Calretinin 22kDa
Specificity:Calretinin, no cross-reaction with Calbindin and Calmodulin
Species reactivity:Human, Rat
Product presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 200-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:Ig
Antibody's clone:6B3
Product is supplied as:liquid concentrate
Recommended concentration for use:1:40-1:50
Protein concentration:N/A
Positive control:Cerebral cortex, pleural mesothelioma
Pretreatment:Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)
Tested applications:IHC(C, P), Immunoblotting
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
Storage method:Store at 2-8°C
UniProt number:P22676 (CALB2_HUMAN)
Litterature:1. Schwaller B., Buchwald P., Blümcke I., Celio M.R., and Hunziker W. (1994) Charakterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin. Cell Calcium 14; 639-648. 2. Gotzos V., Vogt P., and Celio M.R. (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192; 137-147. 3. Doglioni C., Tos A.P., Laurino L., Iuzzolino P., Chiarelli C., Celio M.R., and Viale G. (1996) Calretinin: a novel immunohistochemical marker for mesothelioma. Am. J. Surg. Pathol. 20; 1037-1046. 4. Zimmermann L., and Schwaller B. (2002) Monoclonal antibodies recognizing epitopes of calretinins: dependence on Ca2+-binding status and differences in antigen accessibility in colon cancer cells. Cell Calcium 31; 13-25.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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