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Mouse Monoclonal IgG1 Anti-CML (N-Epsilon)-Carboxymethyl-Lysine : Biotin conjugate [CMS-10]

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Catalog number:CML011BIO
Name:Mouse Monoclonal IgG1 Anti-CML (N-Epsilon)-Carboxymethyl-Lysine : Biotin conjugate [CMS-10]
Size:50 µg
Price:1074.00
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Availability:Please contact us to check the availability
Immunogen:N-epsilon-(Carboxymethyl)-Lysine (CML) conjugated to KLH
Specificity:Reaction with CML-HSA and free CML in competitive ELISA, no reactivity with CEL
Species reactivity:not relevant
Product presentation:Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace (Casein-containing solution, Dainippon Co.) and 0.1% ProClin (Rohm & Haas) as a preservative
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1
Antibody's clone:CMS-10
Product is supplied as:liquid concentrate
Recommended concentration for use:ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml
Protein concentration:0.25 mg/ml
Tested applications:IHC, ELISA
Recommended secondary reagent:Streptavidin:HRPO conjugate (Art. No. ZU054) or alkaline phosphatase conjugate (Art.-Nr. ZU051) in combination with a suitable substrate.
Storage method:Store at -20°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. Dunn J.A., Patrick J.S., Thorpe S.R., Baynes J.W. (1989): Oxidation of glycated proteins: age-dependent accumulation of N-epsilon-(carboxymethyl) lysine in lens proteins. Biochemistry. 28: 9464-9468. 3. Fu M.X., Requena J.R., Jenkins A.J., Lions T.J., Baynes J.W., Thorpe S.R. (1996): The advanced glycation end product, N-epsilon-(carboxymethyl) lysine, is a product of both lipid peroxidation and glycoxidation reactions. J.Biol.Chem.271: 9982-9986
Tips:Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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