Mouse Monoclonal IgG1 Anti-Collagen Type IV, human [24D1]

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Catalog number:CO60411
Name:Mouse Monoclonal IgG1 Anti-Collagen Type IV, human [24D1]
Size:0.1 ml
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Availability:Please contact us to check the availability
Immunogen:Purified collagen type IV from human placenta
Specificity:Human collagen type IV 100%, human collagen types I, II, III, and V
Species reactivity:Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV
Product presentation:0.1 ml affinity purified antibody prod. in nude mice; lyophilized from phosphate buffered solution; no BSA and preservative added!
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1
Antibody's clone:24D1
Product is supplied as:purified, lyophilized
Recommended concentration for use:IFA ≥ 1:320, IHC(P) ≥ 1:4000, ELISA ≥ 1:4000 (OD ≥ 500)
Protein concentration:app. 1 mg/ml
Positive control:Human skin, placenta or lens capsule
Pretreatment:After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Tested applications:IHC(P), IFA, ELISA, RIA, IB/WB
Recommended secondary reagent:Anti-mouse IgG-conjugates, e.g. Anti-mouse IgG:FITC (Art. No. FI-2000) or anti-mouse IgG:DyLight488 (Art. No. DI-2488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7402).
Storage method:Store at -20°C
UniProt number:PGo to UniProt/SwissProt2462 (CO4A1_HUMAN) ; PGo to UniProt/SwissProt8572 (CO4A2_HUMAN); P294Go to UniProt/SwissProtGo to UniProt/SwissProt (CO4A5_HUMAN);
Litterature:1. Guerret S, Govignon E, Hartmann DJ, Ronfard V. (2003) Long-term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Repair Regen. Jan-Feb;11(1):35-45. 2. Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V. (2009) Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Gene:Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Properties:Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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