Bioxys

Mouse Monoclonal IgG1 Anti-Cytokeratin (High Molecular Weight) 34ßE12 [34ßE12]

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Catalog number:CK134
Name:Mouse Monoclonal IgG1 Anti-Cytokeratin (High Molecular Weight) 34ßE12 [34ßE12]
Size:1 ml
Price:343.00
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Availability:Please contact us to check the availability
Immunogen:Cytokeratin extract of human stratum corneum
Specificity:High molecular weight cytokeratins (56-57 and 66-68 kDa)
Species reactivity:Human, monkey, rabbit, horse
Product presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1
Antibody's clone:34ßE12
Product is supplied as:liquid concentrate
Recommended concentration for use:1:10 - 1:20
Protein concentration:S/N
Positive control:Benign prostate
Pretreatment:Unmasking fluid E (Art. No. DE006) or unmasking fluid G (Art. No. DE007) at high temperature is recommended for the use on formaldehyde fixed tissue.
Tested applications:IHC(C, P)
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Storage method:Store at 2-8°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Gown A.M. and Vogel A.M. (1982) Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J. Cell Biol. 95; 414 ff 3. Gown A.M. and Vogel A.M. (1984) Monoclonal antibodies to intermediate filament proteins of human cells II: Distribution of filament proteins in normal human tissue. Am. J. Pathol. 114; 309 ff 4. Yang X.J., Lecksell K., Gaudin P., and Epstein J.I. (1999) Rare expression of high-molekular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer. Am. J. Surg. Pathol. 23(2); 147-152.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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