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c-Raf/Raf1 Antibody, ALEXA FLUOR 488

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About product

Catalog number:bs-1703R-A488
Name:c-Raf/Raf1 Antibody, ALEXA FLUOR 488
Size:100ul
Price:332.75
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Extra details

Long name:c-Raf/Raf1 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as:Anti-c-Raf/Raf1 PAb ALEXA FLUOR 488
Category:Conjugated Primary Antibodies
Conjugated with:ALEXA FLUOR® 488
Host Organism:Rabbit (Oryctolagus cuniculus)
Target Antigen:c-Raf/Raf1
Specificity:This is a highly specific antibody against c-Raf/Raf1.
Modification:Unmodified
Modification Site:None
Clonality:Polyclonal
Clone:Polyclonal antibody
Concentration:1ug per 1ul
Immunogen range:25-60/648
Subcellular location:Cytoplasm, Nucleus, Mitochondrion, Cell membrane
Source:This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human c Raf
Gene ID Number:5894
Swiss Prot:P04049
Tested applications:IF(IHC-P)
Recommended dilutions:IF(IHC-P)(1:50-200)
Crossreactivity:Human, Mouse, Rat
Cross-reactive species details:Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen:Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Purification:Purified by Protein A.
Storage conditions:Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission:499nm/519nm
Synonyms:NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; RAF1; RAF
Properties:For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the c-Raf/Raf1 Antibody, has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation:Alexa Fluor
French translation:anticorps

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