Bioxys

Rabbit Anti-Bovine IgM Antibody (H+L), Biotin Conjugated

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About product

Catalog number:bs-0327R-Biotin
Name:Rabbit Anti-Bovine IgM Antibody (H+L), Biotin Conjugated
Size:0.2ml
Price:121.00
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Extra details

Category:Conjugated Secondary Antibody
Conjugation:Biotin
Host Organism:Rabbit
Target antigen:Bovine IgM
Modification:Unmodified
Modification site:None
Clonality:Polyclonal antibody
Clone:Polyclonal antibody
Concentration:1ug/ul
Tested applications:WB, ELISA, IHC-P, IHC-F
Recommended dilutions:WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
Cross reactivity:Bovine
Background of the target antigen:Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.
Purification method:This antibody has been purified via Protein A.
Storage conditions:Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Additional conjugation:Biotinylated
Additional isotype:IgM
About:Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:Oryctolagus cuniculus
French translation:anticorps

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