Bioxys

Biotin Fluorescent Particles, PAK Blue, 0.1% w/v, 7.0-7.9 um, 5 mL

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About product

Catalog number:07-TFP-7067-5
Name:Biotin Fluorescent Particles, PAK Blue, 0.1% w/v, 7.0-7.9 um, 5 mL
Size:5 mL
Price:273.00
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Extra details

Concentration:0.1% w/v
Nominal size:7.0-7.9 um
Description:What makes our Fluorescent Particles excellent tools for bioimaging and biosensing is their stability and uniformal fluorescence. We can supply fluorescent particles with functional groups for covalent binding of proteins, peptides and antibodies. We can supply particles covered with functuonal groups such as Amino groups, Carboxyl groups, Dimethylamino groups, etc. The SPHERO fluorescent microparticles are produced by staining polystyrene particles with a fluorophore solution or by polymerizing a fluorophore in styrene in the presence of polystyrene core particles. This way an enormous variety of fluorescent particles can be made, with a large range in terms of size, fluorophores, intensity of the fluorescence and various surface functional groups. The fluorophores of our SPHERO fluorescent particles are NOT soluble in water which gives them a great deal of stability. If strored properly, the fluorophores that are incorporated in the particles will not blanch and lose their colour and/or intensity of fluorescence.
Flow cytometer type:These particles can be used for calibration with BD FACSes like FACSCalibur, FACSCanto, Beckman Coulter Flow Cytometer, Becton Dickinson FACScan, Becton Dickinson Flow Cytometers, Miltenyi cytometer, Millipore Flow Cytometers and Millipore Guava.
Technical datasheet:Contact us by phone or email to receive the datasheet or any other useful technical file
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
Conjugation:Biotinylated,Blue Substrate
Additional description:Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.

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