Annexin V Apoptosis Detection Kit with PI-Biotin

View details of this awesome product

About product

Catalog number:ANXVKB-100T
Name:Annexin V Apoptosis Detection Kit with PI-Biotin
Size:100 TEST
Go to shop   

Extra details

Description:Annexin V Apoptosis Detection Kit with PI-Biotin is a kit used for the identifacation and detection of apoptotic and necrotic cells, this Kit was conjugated with Biotin format and reported for use in flow cytometric analysis, it reacts with Annexin V proteins, with Propidium Iodide Solution (PI) helps to make the difference between apoptic cells and necrotic, used also as a dead cell exclusion dye.
Other name:Annexin A5 Apoptosis Detection Kit
Application:Flow Cytometry Method
Reactivity:All mammalian
Formulation:Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
Expiration:After 1 year
Storage:2-8°C, store in dark, do not freeze
Remark:For Recherche Use Only.
Additional description:This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Other suggested products