BAM, Beta-amylase, Biotin conjugated

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Catalog number:AS09 380
Name:BAM, Beta-amylase, Biotin conjugated
Size:1 ml (10 mg/ml)
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Extra details

Available ordering format:Lyophilized in PBS labelled with biotin
Immunogen:Beta amylase isolated and purified from sweet potato Q94EU9
Raised in:Rabbit
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA), immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 379 | anti-beta amylase antibodyPlant and algal protein extraction bufferSecondary antibodies
Recommended dilutions for use:1: 1000 to 1: 4000 for techniques listed above
Molecular weight (expected | аpparent):60 kDa
Verified reactivity:Solanum tuberosum
Possible reactivity:dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis, Vitis vinifera, trees: Populus trichocarpa, Physcomitrella patens
No reactivity:n.a.
Supplementary information:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
References:not available at the moment
Scientific context:Beta amylase (EC catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
Notes:biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
Protein number:Q94EU9
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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