Alpha, Alpha-mannosidase, Biotin conjugated

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About product

Catalog number:AS09 453
Name:Alpha, Alpha-mannosidase, Biotin conjugated
Size:1 ml
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Extra details

Available ordering format:Lyophilized in phosphate buffer saline
Immunogen:native alpha-mannosidase purified from jack beans
Raised in:Rabbit
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 452 | anti-alpha-mannosidase (total IgG)AS09 650 | anti-alpha-mannosidase (affinity purified)Plant and algal protein extraction bufferSecondary antibodies
Recommended dilutions for use:1: 1000 - 1: 100 000 for techniques listed above
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:Canavalia ensiformis (common jack-bean)
Possible reactivity:n.a.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:Biotin/IgG protein molar ration is approximately 1.4. No foreign proteins are added.
References:to be added when available
Scientific context:Alpha-mannosidase cleaves the alpha form of mannose.
Notes:Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Description:The Alpha, Alpha-mannosidase, conjugated is a α- or alpha protein sometimes glycoprotein present in blood.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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