Oxalate, Oxalate oxidase, Biotin conjugated

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Catalog number:AS09 519
Name:Oxalate, Oxalate oxidase, Biotin conjugated
Size:1 ml (10 mg/ml)
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Extra details

Available ordering format:Lyophilized in phosphate buffer saline, labelled with biotin
Immunogen:native oxalate oxidase have been purified from barley seedlings
Raised in:Rabbit
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 518 | anti-oxalate oxidase antibodyPlant protein extraction bufferSecondary antibodies
Recommended dilutions for use:1: 1000 to 1: 4000 for techniques listed above
Molecular weight (expected | аpparent):21 kDa
Verified reactivity:Hordeum vulgare
Possible reactivity:Triticum aestivum, Oryza sativa
No reactivity:n.a.
Supplementary information:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
References:not available at the moment
Scientific context:Oxalate oxidase (EC= belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
Notes:biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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