AGO1, argonaute 1 (40 µg, Biotin conjugated)

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Catalog number:AS09 527B
Name:AGO1, argonaute 1 (40 µg, Biotin conjugated)
Size:40 µg
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Extra details

Available ordering format:Liquid, conjugated to biotin.
Immunogen:N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR:At1g48410.
Raised in:Rabbit
Purification:Affinity purified serum in PBS pH 7.4.
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Verified applications:western blot (WB)
Connected products:AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies
Recommended dilutions for use:1: 1000 (WB)
Molecular weight (expected | аpparent):116.4 | 130 kDa
Verified reactivity:Arabidopsis thaliana
Possible reactivity:Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera
No reactivity:Chlamydomonas reinhardtii
Supplementary information:AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
References:To be added when available, antibody released in November 2015.
Scientific context:AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.
Notes:Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method
Protein number:O04379
TAIR number:AT1G48410
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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