Bromelain, Bromelain, Biotin conjugated

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About product

Catalog number:AS09 553
Name:Bromelain, Bromelain, Biotin conjugated
Size:1 ml
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Extra details

Available ordering format:Lyophilized in phosphate buffer saline
Immunogen:native bromelain isolated and purified from Ananas comosus
Raised in:Rabbit
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 552 | anti-bromelainAS09 554 | anti-bromelain (affinity purified)AS09 546 | anti-PPI | papain (biotinylated)AS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction buffer
Recommended dilutions for use:1: 5000 - 1: 50 000 for techniques listed above
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:Ananas comosus
Possible reactivity:Ananas comosus
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
References:to be added when available
Scientific context:Bromelain (EC= is a cysteine protease isolated from pineapple stem.
Notes:Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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