PLD, phospholipase D, Biotin conjugated

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Catalog number:AS09 557
Name:PLD, phospholipase D, Biotin conjugated
Size:1 ml
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Available ordering format:Lyophilized in phosphate buffer saline
Immunogen:phospholipase D isolated and purified from Brassica oleracea
Raised in:Rabbit
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 556 | anti-PLD phospholipase D AS09 558 | anti-PLD phospholipase D (affinity purified)Plant protein extraction buffer
Recommended dilutions for use:1: 1000 - 1: 20 000 for techniques listed above
Molecular weight (expected | аpparent):92 kDa
Verified reactivity:Brassica oleracea
Possible reactivity:dicots including: Arabidopsis thaliana, Cucumis melo, Ricinus communis, trees: Populus balsamifera
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
References:to be added when available
Scientific context:Phospholipase D (EC= is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
Notes:Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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