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3-hyrdoxybutyrate, dehydrogenase, Biotin conjugated

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About product

Catalog number:AS09 563
Name:3-hyrdoxybutyrate, dehydrogenase, Biotin conjugated
Size:1 ml
Price:507.00
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Extra details

Available ordering format:Lyophilized in phosphate buffer saline
Immunogen:3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides
Raised in:Rabbit
Clonality:Polyclonal
Clone:Polyclonal
Purification:Purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Connected products:AS09 562 | anti-3-hydroxybutyrate dexydrogenaseAS09 564 | anti-3-hydroxybutyrate dexydrogenase (affinity purified)Plant protein extraction buffer
Recommended dilutions for use:1: 100 - 1: 1000 for techniques listed above
Molecular weight (expected | аpparent):26.9 kDa
Verified reactivity:Rhodopacter shpaeroides
Possible reactivity:Sagittula stellata, Silicibacter sp.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
References:to be added when available
Scientific context:3-hydroxybutyrate dehydrogenase (EC=1.1.1.30) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase.
Notes:Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated

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