Goat, L), Biotin conjugated

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About product

Catalog number:AS09 610
Name:Goat, L), Biotin conjugated
Size:2 mg
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Extra details

Available ordering format:Liquid
Immunogen:Purified hen IgY, whole molecule
Raised in:Goat
Purification:Purified hen IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
Verified applications:ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Connected products:AS09 603 | Goat anti-hen IgY (H&L) HRP conjugatedAS09 606 | Goat anti-hen IgY (H&L) AP conjugated
Recommended dilutions for use:1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:hen IgY heavy and light chains (H&L)
Possible reactivity:hen IgY heavy and light chains (H&L)
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
References:to be added when available
Scientific context:Goat anti-hen IgY is a secondary antibody conjugated to biotin which binds to hen IgY immunoglobulin in immunological assays.
Notes:Antibody concentration is 3.86 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name:Capra aegagrus hircus

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