Bioxys

Donkey, L), Biotin conjugated

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Catalog number:AS09 611
Name:Donkey, L), Biotin conjugated
Size:2 mg
Price:312.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified sheep IgG, whole molecule
Raised in:Donkey
Clonality:Polyclonal
Clone:Polyclonal
Purification:Purified donkey IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
Verified applications:ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Connected products:AS09 604 | Donkey anti-sheep IgG (H&L) HRP conjugated
Recommended dilutions for use:1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:sheep IgG heavy and light chains (H&L)
Possible reactivity:sheep IgG heavy and light chains (H&L)
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
References:to be added when available
Scientific context:Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.
Notes:Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
Latin name:Equus africanus asinus

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