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Rabbit, Fc, Biotin conjugated, min. cross-reactivity to human serum

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About product

Catalog number:AS10 1143
Name:Rabbit, Fc, Biotin conjugated, min. cross-reactivity to human serum
Size:1 mg
Price:363.00
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Extra details

Available ordering format:Liquid
Immunogen:to be determined
Raised in:Rabbit
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Verified applications:Contact Gentaur support center
Connected products:other biotinylated secondary antibodies to goat IgG
Recommended dilutions for use:Needs to be determined by end user for all immunoassay applications.
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Refer to PubMed
Scientific context:Rabbit anti-goat IgG Fc, biotin conjugated, is a secondary antibody which binds to goat IgG Fc part in immunological assays. Antibody is adsorbed against human serum and affinity purified using solid phase goat IgG.
Notes:This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on goat immunoglobulins or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation:Biotinylated
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C
Latin name:Oryctolagus cuniculus
Description:Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Group:Sera

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