Bioxys

Goat, Human kappa chain, Biotin conjugated

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Catalog number:AS10 1404
Name:Goat, Human kappa chain, Biotin conjugated
Size:0.5 mg
Price:363.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified human Kappa (k) Chain BAA37169.1
Raised in:Goat
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:2-8 °C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Verified applications:Contact Gentaur support center
Connected products:other secondary antibodies to human immunoglobulins
Recommended dilutions for use:This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:2000 - 1:20 000 for ELISA/Western blot 1:500 - 1:5 000 for Immunohistochemistry
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Reinhart et al. (2014). In search of expression bottlenecks in recombinant CHO cell lines-a case study. Appl Microbiol Biotechnol. 2014 Feb 21.
Scientific context:Goat anti-human kappa (k) chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation:Biotinylated
Latin name:Capra aegagrus hircus

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