Bioxys

Goat, Goat anti-biotin, HRP conjugated

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About product

Catalog number:AS10 1454
Name:Goat, Goat anti-biotin, HRP conjugated
Size:1 mg
Price:312.00
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Extra details

Available ordering format:Lyophilized
Immunogen:Biotin
Raised in:Goat
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified IgG
How to reconstitute:For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage condition:Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Verified applications:Contact Gentaur support center
Connected products:other secondary antibodies against biotin
Recommended dilutions for use:This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:5 000 for Immunohistochemistry 1:200 - 1:5000 for ELISA/Western blot
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Refer to PubMed
Scientific context:Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation:Biotinylated,HRP Conjugated
Latin name:Capra aegagrus hircus

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