Bioxys

Goat, Goat anti-biotin, TRITC conjugated

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About product

Catalog number:AS10 1455
Name:Goat, Goat anti-biotin, TRITC conjugated
Size:1 mg
Price:312.00
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Extra details

Available ordering format:Liquid
Immunogen:Biotin
Raised in:Goat
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Verified applications:Contact Gentaur support center
Connected products:other secondary antibodies against biotin
Recommended dilutions for use:This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Refer to PubMed
Scientific context:Goat anti-biotin is a secondary antibody conjugated to TRITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
Latin name:Capra aegagrus hircus

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