Bioxys

Rabbit, L), Biotin conjugated

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About product

Catalog number:AS10 1522
Name:Rabbit, L), Biotin conjugated
Size:1 mg
Price:294.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified rat IgG (H&L)
Raised in:Rabbit
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified Rabbit IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Verified applications:Contact Gentaur support center
Connected products:other biotinylated secondary antibodies against rat IgG (H&L)
Recommended dilutions for use:This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:2000 - 1:20 000 for ELISA/Western blot 1:500 - 1:5 000 for Immunohistochemistry
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Refer to PubMed
Scientific context:Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated biotin, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:Oryctolagus cuniculus

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