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Rabbit, L), Biotin conjugated, min. cross-reactivity to human serum

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Catalog number:AS10 1529
Name:Rabbit, L), Biotin conjugated, min. cross-reactivity to human serum
Size:1 mg
Price:294.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified rat IgG (H&L)
Raised in:Rabbit
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified Rabbit IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Verified applications:Contact Gentaur support center
Connected products:other biotinylated secondary antibodies against rat IgG (H&L)
Recommended dilutions for use:This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:2000 - 1:20 000 for ELISA/Western blot, 1:500 - 1:5 000 for Immunohistochemistry.
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:Refer to PubMed
Scientific context:Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation:Biotinylated
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C
Latin name:Oryctolagus cuniculus
Description:Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Group:Sera

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