Goat, L), Biotin conjugated

Browse details of product

About product

Catalog number:AS10 655
Name:Goat, L), Biotin conjugated
Size:2 mg
Go to shop   

Extra details

Available ordering format:Liquid
Immunogen:Purified Guinea pig IgG, whole molecule
Raised in:Goat
Purification:Affinity purified IgG
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store undiluted liquid at 2-8°C. Shelf life is 1 year from date of receipt.
Verified applications:ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Connected products:no related to other products
Recommended dilutions for use:1: 2000 - 20 000 (ELISA) and (WB), 1: 500 -1: 5000 (IHC)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:guinea pig IgG heavy and light chains (H&L)
Possible reactivity:guinea pig IgG heavy and light chains (H&L)
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis.This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
References:to be added when available
Scientific context:Goat anti-guinea pig IgG is a secondary antibody conjugated to biotin which binds to all guinea pig immunoglobulins in immunological assays.
Notes:biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name:Capra aegagrus hircus

Other suggested products