Biotin, Biotin carboxyl carrier subunit of chloroplast ACCase

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About product

Catalog number:AS15 2881
Name:Biotin, Biotin carboxyl carrier subunit of chloroplast ACCase
Size:100 µl
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Extra details

Available ordering format:Lyophilized
Immunogen:Purified native protein from Pisum sativum
Raised in:Guinea pig
How to reconstitute:See included datasheet or contact our support service
Storage condition:For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Verified applications:western blot (WB)
Connected products:AS15 2880 | anti-ACCase subunit beta | acetyl-coenzyme A, carboxylase (subunit beta) rabbit antibodySecondary antibodies
Recommended dilutions for use:1:2000 with ECL (WB)
Molecular weight (expected | аpparent):29.6 kDa
Verified reactivity:Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
Possible reactivity:Hordeum vulgare, Nicotiana tabacum
No reactivity:no confirmed exceptions from predicted reactivity are currently known
Supplementary information:Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.
References:to be added when available, antibody released in December 2015
Scientific context:Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
Protein number:F4KE21
TAIR number:AT5G16390
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description:A carrier can hold protected the subject it is supporting for the period needed by its application as stated by agisera.

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