Goat, L), Biotin conjugated

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Catalog number:AS16 3125
Name:Goat, L), Biotin conjugated
Size:1 ml
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Extra details

Available ordering format:Lyophilized in PBS, pH 7.2
Immunogen:Purified normal IgG isolated from pooled dog serum
Raised in:Goat
Purification:Protein G purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.
Verified applications:dot-blot (Dot), ELISA (ELISA), immunocytochemistry (ICC), immunohistochemistry (paraffin) (IHC), western blot (WB)
Connected products:other biotin conjugated secondary antibodies to dog IgGSecondary antibodies
Recommended dilutions for use:1:100 - 1:500 (ICC, IHC), 1:1 000 and 1:10 000 (ELISA) The optimal working dilution should be established by titration by the end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:dog IgG
Possible reactivity:to be determined
No reactivity:This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Supplementary information:Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
References:to be added when available
Scientific context:Goat anti-Dog IgG (H&L), Biotin conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name:Capra aegagrus hircus

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