Goat, Fc specific, Biotin conjugated

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Catalog number:AS16 3129
Name:Goat, Fc specific, Biotin conjugated
Size:1 ml
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Extra details

Available ordering format:Lyophilized in PBS, pH 7.2
Immunogen:Purified normal IgM isolated from pooled dog serum
Raised in:Goat
Purification:Protein G purified IgG
How to reconstitute:For reconstitution please add 1 ml of sterile destilled water.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.
Verified applications:dot-blot (Dot), ELISA (ELISA), immunocytochemistry (ICC), immunohistochemistry (paraffin) (IHC), western blot (WB)
Connected products:other Biotin conjugated secondary antibodies to dog IgMSecondary antibodies
Recommended dilutions for use:1:500-4000 (ELISA), 1:100-250 (IHC and ICC)The optimum working dilution should be established by titration by end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:dog IgM, Fc part
Possible reactivity:to be determined
No reactivity:This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Supplementary information:Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail. No preservative added.
References:to be added when available, antibody released in January 2016.
Scientific context:Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Latin name:Capra aegagrus hircus

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