Rabbit, L), Biotin conjugated

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Catalog number:AS16 3249
Name:Rabbit, L), Biotin conjugated
Size:2.0 mg
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Extra details

Available ordering format:Liquid
Immunogen:Purified Bovine IgG, whole molecule
Raised in:Rabbit
Purification:Affinity purified
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store at 2-8 °C.
Verified applications:ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Connected products:other anti-Bovine secondary antibodies conjugated to biotin
Recommended dilutions for use:1:2 000-1:20 000 (ELISA), 1:500-1:5 000 (IHC), 1:2 000-1:20 000 (WB)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:Bovine IgG (H&L)
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at
References:To be added when available, antibody released in April 2016.
Scientific context:Rabbit anti-Bovine IgG (H&L), Affinity purified is a secondary antibody, conjugated to Biotin, which bind to Bovine IgG (H&L) in immunological assays.
Notes:Affinity purified antibody is> 95% based on SDS-PAGE.Affinity purified using solid phase Bovine IgG (H&L).Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.0.05% (w/v) Sodium Azide is added as preservative.Based on IEP, this antibody reacts with: · heavy (gamma) chains on bovine IgG · light chains on all bovine immunoglobulins. Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins.
Protein number:DAA31645
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:Oryctolagus cuniculus

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