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Goat, L), Biotin conjugated, min. cross-reactivity to bovine, goat, mouse or rabbit serum proteins

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Catalog number:AS16 3325
Name:Goat, L), Biotin conjugated, min. cross-reactivity to bovine, goat, mouse or rabbit serum proteins
Size:1.0 mg
Price:266.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified Human IgG, whole molecule
Raised in:Goat
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store at 2-8 °C.
Verified applications:Flow cytometry (FACS), Immunomicroscopy (IM)
Connected products:other anti-human secondary antibodies
Recommended dilutions for use:1:20 - 1:2 000 (FACS and IM)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:Human IgG (H&L)
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:To be added when available, antibody released in April 2016.
Scientific context:Goat anti-Human IgG (H&L), Biotin conjugated, min. cross-reactivity to bovine, goat, mouse or rabbit serum proteins is a secondary antibody conjugated to biotin, which binds to human IgG (H&L) in immunological assays.
Notes:Antibody purity is> 95% based on SDS-PAGE.Affinity purified using solid phase Human IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgG - light chains on all human immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum immunoglobulins - serum proteins from bovine, goat, mouse or rabbit .
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
Latin name:Capra aegagrus hircus,Mus musculus,Oryctolagus cuniculus
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C
Description:Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Group:Sera

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