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Goat, Fc, Biotin conjugated, min. cross-reactivity to bovine, horse or human serum proteins

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About product

Catalog number:AS16 3479
Name:Goat, Fc, Biotin conjugated, min. cross-reactivity to bovine, horse or human serum proteins
Size:2.0 mg
Price:312.00
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Extra details

Available ordering format:Liquid
Immunogen:Purified Mouse IgG Fc
Raised in:Goat
Clonality:Polyclonal
Clone:Polyclonal
Purification:Affinity purified
How to reconstitute:See included datasheet or contact our support service
Storage condition:Store at 2-8 °C.
Verified applications:Flow cytometry (FACS), Immunomicroscopy (IM)
Connected products:other anti-mouse secondary antibodies
Recommended dilutions for use:1:20 - 1:2 000 (FACS and IM)
Molecular weight (expected | аpparent):see datasheet
Verified reactivity:Mouse IgG Fc
Possible reactivity:to be determined
No reactivity:to be determined
Supplementary information:Contact Gentaur support center at info@gentaur.com
References:To be added when available, antibody released in April 2016.
Scientific context:Goat anti-Mouse IgG Fc, Biotin conjugated, min. cross-reactivity to bovine, horse or human serum proteins is a secondary antibody conjugated to biotin, which binds to mouse IgG Fc in immunological assays.
Notes:Antibody purity is> 95% based on SDS-PAGE.Affinity purified using solid phase Mouse IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on mouse IgG . Based on IEP, no reactivity is observed to: - non-immunoglobulin mouse serum proteins - light chains on all mouse immunoglobulins - mouse IgG, F(ab)'2 fragment - serum proteins from bovine, horse, or human .
Protein number:Refer to NCBI
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation:Biotinylated
Latin name:Capra aegagrus hircus
Description:Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Group:Sera
About:Serum should be stored at +5°C

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