CO4, Complement component C4, Biotin conjugated

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About product

Catalog number:IMS06-032-304
Name:CO4, Complement component C4, Biotin conjugated
Size:100 µl
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Extra details

Available ordering format:Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Immunogen:full length native protein P0C0L4 purified from human plasma
Raised in:Hen
Purification:Affinity purified IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications:Western blot (WB)
Connected products:IMS02-032-303 | anti-Complement component C4IMS01-031-305 | anti-Complement component C4cIMS01-066-309 | anti-Complement factor H
Recommended dilutions for use:use at an assay dependent dilution (WB)
Molecular weight (expected | аpparent):193 kDa
Verified reactivity:human
Possible reactivity:human
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum or human C4. Only one precipitin arc in immunoelectrophoresis against normal human serum or human C4. Specific against human C4 alpha and beta chains in electroblotting when tested against normal human serum.
References:to be added when available
Scientific context:Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Notes:affinity purified on human C4 agaroseconcentration: 1 mg/ml
Protein number:P0C0L4
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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