BSA, Bovine serum albumin, Biotin conjugated

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About product

Catalog number:IMS06-035-327
Name:BSA, Bovine serum albumin, Biotin conjugated
Size:100 µl (1 µg/µl)
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Extra details

Available ordering format:Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Immunogen:full length native protein, P02769
Raised in:Hen
Purification:Affinity purified IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications:Western blot (WB), immunodifusion (ID)
Connected products:AS99 002 | anti-BSA rabbit antibodyAS02 023 | anti-BSA hen antibodyIMS02-035-326 | anti-BSA antibody
Recommended dilutions for use:1: 5 000 (WB)
Molecular weight (expected | аpparent):67 kDa
Verified reactivity:bovine
Possible reactivity:goat, sheep
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:only one precipitation arc in immunodiffusion against normal bovine serum or bovine serum albumin. Only one precipitin arc in immunoelectrophoresis against normal bovine serum or bovine serum albumin.
References:to be added when available
Scientific context:Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids and thyroid hormones, and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Notes:affinity purified IgY on bovine serum albumin agarose; labelled with biotin
Protein number:P02769
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description:Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
About:Serum should be stored at +5°C

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