Fibrinogen, Fibrinogen, Biotin conjugated

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Catalog number:IMS06-038-312
Name:Fibrinogen, Fibrinogen, Biotin conjugated
Size:100 µl (1mg/ml)
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Available ordering format:Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Immunogen:Purified, full length native fibrinogen Q9UE34
Raised in:Hen
Purification:Affinity purified IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications:Western blot (WB), immunodifusion (ID)
Connected products:IMS09-038-335 Fibrinogen | labelled with fluorescien
Recommended dilutions for use:1: 5000 (WB)
Molecular weight (expected | аpparent):75 kDa
Verified reactivity:human
Possible reactivity:bovine, mouse, porcine, rabbit, rat
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:only one precipitation arc in immunodiffusion and immunoelectrophoresis against normal human plasma or human fibrinogen
References:Kinzebach et al. (2013). Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol. 2013 Oct 30;14(1):48.
Scientific context:Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N-terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel et al. 2000; Rapold et al. 1989).
Protein number:Q9UE34
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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