EPO, Erythropoietin, Biotin conjugated

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Catalog number:IMS06-114-311
Name:EPO, Erythropoietin, Biotin conjugated
Size:100 µl (15 mg/ml)
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Extra details

Available ordering format:Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Immunogen:Purified, full length recombinant protein P01588
Raised in:Hen
Purification:Purified IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications:Western blot (WB)
Connected products:IMS01-114-340 | anti-EPO | Erythropoietin, chicken antibody
Recommended dilutions for use:1: 5 000 with standard ECL (WB)
Molecular weight (expected | аpparent):21 | 34 kDa
Verified reactivity:human
Possible reactivity:cat, dog, porcine, rat
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:detects a band of approximately 34 kDa due to glycosylation of EPO (predicted molecular weight is 21 kDa (Swiss-prot))
References:to be added when available
Scientific context:Human erythropoietin (EPO) is an acidic glycoprotein hormone with a molecular mass of 21 kDa. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis.
Notes:the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation. Labelled with biotin.
Protein number:P01588
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

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