G-CSF, Granulocyte colony-stimulating factor, Biotin conjugated

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Catalog number:IMS06-119-317
Name:G-CSF, Granulocyte colony-stimulating factor, Biotin conjugated
Size:100 µl
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Extra details

Available ordering format:Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Immunogen:purified full length recombinant human protein P09919
Raised in:Hen
Purification:Affinity purified IgY
How to reconstitute:See included datasheet or contact our support service
Storage condition:store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications:Western blot (WB)
Connected products:IMS01-119-316 | Granulocyte colony-stimulating factorIMS01-120-318 | Granulocyte-macrophage colony-stimulating factor
Recommended dilutions for use:1: 5 000 with standard ECL (WB)
Molecular weight (expected | аpparent):22 kDa
Verified reactivity:human
Possible reactivity:bovine, cat
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Supplementary information:to be added when available
References:to be added when available
Scientific context:Granulocyte colony-stimulating factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
Notes:the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin.Total IgY concentration for this antibody is 15 mg/ml
Protein number:P09919
TAIR number:Refer to NCBI
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description:Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Gene:Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim.

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