Bioxys

MLH1 Polyclonal Antibody

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About product

Catalog number:CAC07641
Name:MLH1 Polyclonal Antibody
Size:50 μg
Price:245.00
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Extra details

Other name:DNA mismatch repair protein Mlh1,MutL protein homolog 1,MLH1,COCA2
Immunogen:Recombinant human DNA mismatch repair protein Mlh1 protein (350-519AA)
Verified reactivity:Human, Mouse
Verified applications:ELISA,WB,IHC. Recommended dilution:WB:1:2000-1:5000,IHC:1:20-1:200
Raised in:Rabbit
Clonality:Polyclonal
Protein number:P40692
Gene number:Please refer to GenBank
NCBI number:Please refer to NCBI
Purity:Protein G purified,>95%
Storage recommendation:Aliquot and store at -20°C. Minimize freezing and thawing.
Use before:1 year
Shipping requirements:Blue ice
Estimated production time:3-7 business days
Supplementary information:Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Notes:For research use only. Not for diagnostic procedures.
Properties:If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group:Polyclonals and antibodies
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation:anticorps

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