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chicken yolk anti-Human IgG (Fab)2 (Biotin) polyclonal IgY antibody

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Catalog number:AHIgGFab-B
Name:chicken yolk anti-Human IgG (Fab)2 (Biotin) polyclonal IgY antibody
Size:500 ug
Price:227.80
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Extra details

Antigen:Human IgG (Fab)2 (Biotin)
Chicken IgY:Gallus gallus domesticus and other IgY antibodies have high affinity and are easy to collect from the yolk. Gallus gallus can produce 20 more than in rabbit polyclonal. The antibody will not bind to rheumatoid factor or protein or Fc regions. This gives very much reduced background very much suited for diagnostic applications. Papain will digest IgY’s to divalent Fab antibody fragments. IgY’s are suited for Elisa kit development, Immunohistochemistry and Western Blot.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:If you buy Antibodies supplied by gallus IgY they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
Test:gallus IgY supplies antibodies that are for research of human proteins.
Conjugation:Biotinylated
Additional isotype: Fab fragment,IgG,IgY antibody
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Group:Polyclonals and antibodies
French translation:anticorps

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