Rabbit anti-Ebola Virus GP BIOTIN-Conjugated Polyclonal Antibody

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Catalog number:494-0301-015-bio
Name:Rabbit anti-Ebola Virus GP BIOTIN-Conjugated Polyclonal Antibody
Size:100 μg
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Extra details

Lot number:1503001
Description:Affinity purified rabbit polyclonal antibody reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant EBOV GP without the transmembrane region (EBOV rGPdTM) in Western blot and ELISA. Purified rabbit polyclonal antibody reactive to EBOV GP was conjugated with biotin and purified from unconjugated biotin by diafiltration. Antibody was conjugated with biotin at a molar ratio of 20:1 (Biotin: antibody)
Virus category:Filoviruses
Type:polyclonal antibody
Viral target:Ebola Virus (EBOV)
Immunogen:Peptide sequence to Ebola virus (EBOV) glycoprotein (GP).
Raised in:Rabbit
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Storage:2-3 weeks +4°C, -20°C long term
Disease:Marburg or Ebola viruses ( EBOV ) need to be handled in BSL4 Biological safety level 4. However the monoclonal and polyclonal antibodies, ELISAs and recombinant proteins do not need any specific safety measure because no active virus is involved. The Zaire Ebola strain is called ZEBOV. The Sudan virus is called SEBOV.
Additional description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:If you buy Antibodies supplied by virus detection they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Group:Polyclonals and antibodies
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by virus detection. Rabbit antibodies are very stable and can be stored for several days at room temperature. virus detection adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:Oryctolagus cuniculus
French translation:anticorps

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