Anti-HSD11B2 (Polyclonal), Biotin

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Catalog number:GENTObs-20186R-Biotin
Name:Anti-HSD11B2 (Polyclonal), Biotin
Size:100 microliters
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Extra details

Type:Conjugated Primary Antibody
Conjugated with:Biotin
Host organism:Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide:HSD11B2
Specificity:This antibody reacts specifically with HSD11B2 Biotin
Modification:No modification has been applied to this antibody
Modification site:None
Clonality:Polyclonal Antibody
Clone:Polyclonal Antibodies
Concentration:1ug per 1ul
Subcellular locations:N/A
Antigen Source:KLH conjugated synthetic peptide derived from human HSD11B2
Gene ID:3291
Swiss Prot:P80365
Applications:WB, IHC-P
Applications with corresponding dilutions:WB(1:100-1000), IHC-P(1:100-500)
Cross reactive species:Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details:No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information:Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Purification method:Purified by Protein A.
Storage:Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission:N/A
Synonyms:AME; AME1; HSD2; HSD11K; SDR9C3; Corticosteroid 11-beta-dehydrogenase isozyme 2; 11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type II; 11-HSD type II; 11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3; HSD11B2
Also known as:HSD11B2 Polyclonal Antibody Biotin
Other name:Anti-HSD11B2 Polyclonal Biotin
Advisory:Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Group:Polyclonals and antibodies
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

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