Bioxys

Rabbit Polyclonal IgG Anti-NF kappa B / p65

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About product

Catalog number:NFK001
Name:Rabbit Polyclonal IgG Anti-NF kappa B / p65
Size:100 µg
Price:0.00
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Extra details

Availability:Please contact us to check the availability
Immunogen:Synthetic peptide from internal domain of NFkB p65
Specificity:NFkB (65kDa)
Species reactivity:Human, mouse, rat (other vertebrates expected, but not tested)
Product presentation:Affinity purified IgG fraction from rabbit serum in PBS containing 0.2% BSA and 0.09% NaN3
Product category:Primary Antibody
Antibody's raised in:Rabbit
Antibody's isotype:IgG
Antibody's clone:polyclonal
Product is supplied as:liquid concentrate
Recommended concentration for use:IHC (2 µg/ml), WB (2-5 µg/ml)
Protein concentration:200 µg/ml
Positive control:Prostate, placenta
Pretreatment:Unmasking with citrate buffer pH 6 10-20 min at 100°C
Tested applications:IHC(P), WB
Recommended secondary reagent:Biotinylated anti-Rabbit IgG (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051) and a suitable substrate.
Storage method:Store at 2-8°C
UniProt number:Go to UniProt/SwissProt
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype:IgG
Group:Polyclonals and antibodies
Latin name:Oryctolagus cuniculus

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